Use of the hemagglutinating virus of Japan (HVJ) envelope as a versatile delivery system for nucleic acids and proteins to leukocytes in vitro.
Use of the hemagglutinating virus of Japan (HVJ) envelope as a versatile delivery system for nucleic acids and proteins to leukocytes in vitro.
Curr Protoc Immunol. 2010 Apr;Chapter 10:Unit 10.17D.1-9
Authors: Kondo Y, Miyata K, Kato F
Abstract
The hemagglutinating virus of Japan (HVJ; also called the Sendai virus) envelope has been developed as a safe and efficient non-viral vector. Because replication and transcription of genomic RNA is inactivated by beta-propiolactone treatment or UV-irradiation, the HVJ envelope is extremely safe. This unit describes the method of transfection of siRNA and protein with the HVJ envelope.
PMID: 20376840 [PubMed - indexed for MEDLINE]
Source: Current Protocols in Immunology - Category: Allergy & Immunology Tags: Curr Protoc Immunol Source Type: research
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