In vivo and ex vivo protocols for measuring the killing of extracellular pathogens by macrophages.

In vivo and ex vivo protocols for measuring the killing of extracellular pathogens by macrophages. Curr Protoc Immunol. 2011 Feb;Chapter 14:Unit 14.19.1-17 Authors: Medina E, Goldmann O Abstract This unit describes a series of in vivo/ex vivo combined protocols for investigating the interactions (adhesion, phagocytosis, and killing) of extracellular bacteria with peritoneal murine macrophages. It includes steps needed for in vivo infection of murine peritoneal macrophages after intraperitoneal inoculation with the pathogen of interest, as well as the measurement of bacteria associated with or truly internalized by these phagocytic cells. Several protocols for the ex vivo measurement of the ability of peritoneal macrophages to kill the microorganisms that have been ingested during the in vivo infection assay are included. PMID: 21400679 [PubMed - indexed for MEDLINE]
Source: Current Protocols in Immunology - Category: Allergy & Immunology Tags: Curr Protoc Immunol Source Type: research