Analysis of gene expression and gene silencing in human macrophages.
Analysis of gene expression and gene silencing in human macrophages.
Curr Protoc Immunol. 2012 Feb;Chapter 14:Unit 14.28.1-23
Authors: Martinez FO
Abstract
This unit describes how to execute a gene expression study with human macrophages. It includes protocols for human macrophage preparation, RNA extraction, real-time PCR analysis, and microarray analysis. The unit also includes a protocol for gene silencing in human macrophages. Altering gene expression can be useful to study the contribution of the gene to macrophage function or even expression of other genes.
PMID: 22314831 [PubMed - indexed for MEDLINE]
Source: Current Protocols in Immunology - Category: Allergy & Immunology Tags: Curr Protoc Immunol Source Type: research