Genome-wide identification of the Na+/H+ exchanger gene family in Lateolabrax maculatus and its involvement in salinity regulation

Publication date: Available online 9 January 2019Source: Comparative Biochemistry and Physiology Part D: Genomics and ProteomicsAuthor(s): Yang Liu, Haishen Wen, Xin Qi, Xiaoyan Zhang, Kaiqiang Zhang, Hongying Fan, Yuan Tian, Yanbo Hu, Yun LiAbstractNa+/H+ exchangers (NHEs) are one of the major groups of transmembrane proteins that play crucial roles in pH homeostasis, cell volume regulation and Na+ transport in animals. In our study, twelve NHEs were identified from transcriptomic and genomic databases of Lateolabrax maculatus. The evolutionary footprint of each NHE gene was revealed via the analysis of phylogenetic tree, copy numbers, exon-intron structures and motif compositions. NHEs harbored a high proportion of α-helices (54.7% to 67.0%) and a low proportion of β-sheets (1.3%) and contained 9–13 transmembrane helices (TM). Results of tissue distribution detection revealed that L. maculatus NHE genes exhibited different expression profiles in a tissue-specific manner under normal physiological conditions. In the main osmoregulatory organ, gill, NHE2c and NHE3 showed significant higher expression comparing with other L. maculatus NHE genes. To gain insight into the potential function of L. maculatus NHE genes in response to salinity changes, we evaluated their expression variation after different salinity treatment (0 ppt, 12 ppt, 30 ppt, 45 ppt). During acute salinity stress experiment, L. maculatus NHE genes were regulated in a gene-specific and time-depende...
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - Category: Genetics & Stem Cells Source Type: research