6 Cell Wall Teichoic Acids in the Taxonomy and Characterization of Gram-positive Bacteria††Dedicated to the memory of Professor L.V. Backinowsky (1938–2011) – friend and colleague, whose inspiration and insight problems contributed to the writing of this chapter.

Publication date: 2011 Source:Methods in Microbiology, Volume 38 Author(s): Natal’ya V. Potekhina , Galina M. Streshinskaya , Elena M. Tul'skaya , Alexander S. Shashkov The use of cell wall teichoic acids as chemotaxonomic markers in the systematics of members of the order Actinomycetales and the genus Bacillus is discussed. Numerous examples show that these polymers are species-specific for representatives of the genera Actinomadura, Agromyces, Brevibacterium, Glycomyces, Nocardioides, Nocardiopsis, Nonomuraea, Streptomyces, and type strains of the B. subtilis group. The structural diversity and abundance of such polymers in Gram-positive bacteria is demonstrated. Methods for the isolation of cell wall teichoic acids and their component analysis as well as chemical and NMR spectroscopic methods for the complete structure elucidation are described.

Source: Methods in Microbiology - October 12, 2014 Category: Microbiology Source Type: research