Chapter 5 Targeted and quantitative metabolomics in bacteria

Publication date: 2012 Source:Methods in Microbiology, Volume 39 Author(s): Hannes Link , Joerg Martin Buescher , Uwe Sauer Targeted metabolomics aims at reliable and sensitive quantification of a subset of metabolites in a cell. Here, we describe our current methods to obtain quantitative intracellular concentrations of a specified set of metabolites in bacteria. The protocol is structured into four steps of the workflow: standardized cultivation, sampling for metabolome analysis, extraction of metabolites and subsequent analysis by mass spectrometry. At the end, we give a brief discussion about the use of metabolite data.

Source: Methods in Microbiology - October 12, 2014 Category: Microbiology Source Type: research