Chapter 4 Imaging fluorescent protein fusions in live bacteria

Publication date: 2012 Source:Methods in Microbiology, Volume 39 Author(s): Geoff Doherty , Karla Mettrick , Ian Grainge , Peter J. Lewis The use of fluorescent protein fusions has revolutionised our understanding of bacterial cell structure. Here we present a brief overview of the molecular toolkits available for the production of functional fluorescent protein fusions in Gram positive and Gram negative organisms. We highlight considerations for performing live cell imaging including equipment requirements and simple approaches to image processing.

Source: Methods in Microbiology - October 12, 2014 Category: Microbiology Source Type: research