Chapter 3 Proteomics From relative to absolute quantification for systems biology approaches

Publication date: 2012 Source:Methods in Microbiology, Volume 39 Author(s): Andreas Otto , Jörg Bernhardt , Michael Hecker , Uwe Völker , Dörte Becher Within the concert of OMICs technologies proteomics is particularly important for systems biology approaches because it provides large-scale, quantitative information on the effectors of cellular processes inside living organisms. For a comprehensive analysis of the proteome 2D-gel- based and gel-free, mass spectrometry-driven methods are available relying on different workflows for identification of proteins and time-resolved analysis of changes in their abundance, post-translational modifications included. In this chapter we describe appropriate sample processing and workflows for relative and absolute protein quantification. For absolute protein quantification we illustrate the AQUA and QConCat approaches. Finally, a workflow for calibration of large scale proteomics data to yield absolute proteomics data is outlined enabling generation of absolute proteomics data on a global scale.

Source: Methods in Microbiology - October 12, 2014 Category: Microbiology Source Type: research