Multidrug Resistance and the Predominance of < b > < i > bla < /i > < /b > < sub > CTX-M < /sub > in < sub > < /sub > Extended Spectrum Beta-Lactamase-Producing Enterobacteriaceae of Animal and Water Origin

The aim of this work was the genetic characterization of cefotaxime-resistant enterobacteria from animals (53 samples), the surface water of rivers (17 samples), and wastewater treatment plants (43 samples) in Tunisia. A total of 48 (42.4%) cefotaxime-resistant isolates were recovered. An extended spectrum beta-lactamase (ESBL) phenotype with a positive double-disk synergy test (DDST) was exhibited by 34 (70.8%) and 14 (29.1%) isolates from water and animal origins, respectively. Isolates from water were identified as:Escherichia coli (n = 17),Hafnia spp. (n = 13),Citrobacter spp. (n = 1),Enterobacter cloacae (n = 1),Klebsiella pneumoniae (n = 1), andK. oxytoca (n = 1). Animal isolates were identified as:E. coli (n = 11),E. cloacae (n = 1),Hafnia spp. (n = 1), andK. pneumoniae (n = 1). PCR investigation ofblaCTX-M,blaTEM, andblaSHV genes showed that amongst the 48 isolates with a positive DDST, 41 (87.5%) carried theblaCTX-M gene, 1 isolate harbored theblaSHV gene, and 1 isolate coharboredblaCTX-M withblaSHV genes. The class 1 and 2 integrons were detected in 27 (56.2%) and 1 (2%) isolates, respectively. Our study showed a significant occurrence of ESBL-producing enterobacteria in animals and aquatic environments with a predominance ofblaCTX-M genes.J Mol Microbiol Biotechnol 2018;28:201 –206
Source: Journal of Molecular Microbiology and Biotechnology - Category: Microbiology Source Type: research