Composition analysis and antioxidant activities of the Rhus typhina L. stem

Publication date: Available online 2 January 2019Source: Journal of Pharmaceutical AnalysisAuthor(s): Ting Liu, Zhaoqin Li, Ruiyun Li, Yue Cui, Yunli Zhao, Zhiguo YuAbstractThe present investigation reports the chemical composition of the Rhus typhina L. stem identified via mass spectrometry and NMR as gallic acid, 1-O-galloyl-β-D-glucose, tryptophan, scopolin, methyl gallate, fustin, quercetin, rutin, 1,2,3,4,6-penta-O-galloyl-β-D-glucose. The antioxidant properties and the chemical composition contents of the Rhus typhina L. stem grown in different regions in China were determined. To determine the antioxidant activity, a total phenolic content analysis, 2, 2-diphenyl-1-picrylhydrazyl radical scavenging activity assay, ferric reducing antioxidant power assay and β-carotene linoleic acid model system were conducted. The results showed that the Rhus typhina L. stem possessed high antioxidant capacities due to its high phenolic content. The contents of the nine isolated compounds were determined by UPLC-ESI-MS/MS. The calibration curves of the nine isolated compounds were linear within the concentration range and the average recoveries were high. The result showed that 1-O-galloyl-β-D-glucose, gallic acid, methyl gallate and 1,2,3,4,6-penta-O-galloyl-β-D-glucose could be the compounds mainly responsible for the antioxidant capacity of the R. typhina L. stem. This reveals that the Rhus typhina L. stem is a good source of antioxidants.

Source: Journal of Pharmaceutical Analysis - January 3, 2019 Category: Drugs & Pharmacology Source Type: research