Horseradish (Moringa oleifera) seed and leaf inclusive diets modulates activities of enzymes linked with hypertension, and lipid metabolites in high-fat fed rats

This study examined the effects of Horseradish leaves and seeds inclusive diets on angiotensin-I converting enzyme (ACE) and arginase activities and lipid [total cholesterol (TC), triglycerides (TGs), low-density lipoprotein cholesterol (LDL-C) and high-density lipoprotein cholesterol (HDL-C)] metabolites in rats fed with high-cholesterol (HC) diet. Animals were divided into seven groups (n = 7) including normal control rats (NC) and untreated hypercholesterolemia rats (HC). Phenolic constituents of the horseradish leaf and seed were determined via high performance liquid chromatography (HPLC-DAD). The result revealed that rats in HC group had higher (p < 0.05) ACE and arginase activities with higher TC, TG and LDL-C levels with a concomitant decrease in HDL-C level. However, dietary supplementation with 2 and 4% Horseradish leaf and seed reversed ACE and arginase activities, TC, TG, and LDL-C and HDL-C levels. HPLC-DAD analysis also revealed the presence of ellagic acid, chlorogenic acid and gallic acid, kaempferol, isoquercitrin, rutin, and quercetin. The enzyme inhibitory and hypocholesterolemic effects exhibited by diets supplemented with horseradish leaf and seed could be associated with their phenolic constituents. These could be the underlying mechanisms of the use of Horseradish in folklore for the management of hypercholesterolemia and its complications.Graphical abstract
Source: PharmaNutrition - Category: Nutrition Source Type: research