Adipose tissue oxylipin profiles vary by anatomical site and are altered by dietary linoleic acid in rats

Publication date: Available online 27 December 2018Source: Prostaglandins, Leukotrienes and Essential Fatty AcidsAuthor(s): Lucien GJ Cayer, Anne M Mendonça, Samantha D Pauls, Tanja Winter, Shan Leng, Carla G Taylor, Peter Zahradka, Harold M AukemaAbstractDietary PUFA and their effects on adipose tissue have been well studied, but oxylipins, the oxygenated metabolites of PUFA, have been sparsely studied in adipose tissue. To determine the oxylipin profile and to examine their potential importance in various adipose sites, female and male rats were provided control, high linoleic acid (LA), or high LA and high α-linolenic acid (LA+ALA) diets for six weeks. Analysis of gonadal (GAT), mesenteric (MAT), perirenal (PAT), and subcutaneous adipose tissues (SAT) revealed higher numbers of oxylipins in MAT and SAT, primarily due to 20-22 carbon cytochrome P450 oxylipins, as well as metabolites of cyclooxygenase derived oxylipins. LA oxylipins made up 75-96% of the total oxylipin mass and largely determined the total relative amounts between depots (GAT>MAT>PAT>SAT). However, when the two most abundant LA oxylipins (TriHOMEs) were excluded, MAT had the highest mass of oxylipins and exhibited the most sex differences. These differences existed despite comparable PUFA composition between depots. Dietary LA increased oxylipins derived from n-6 PUFA, and the addition of ALA generally returned n-6 PUFA oxylipins to levels similar to control and elevated some n-3 oxylipins. These data on o...

Source: Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA) - December 27, 2018 Category: Lipidology Source Type: research