Protein identification using Sorcerer 2 and SEQUEST.

Protein identification using Sorcerer 2 and SEQUEST.
Curr Protoc Bioinformatics. 2009 Dec;Chapter 13:Unit 13.3
Authors: Lundgren DH, Martinez H, Wright ME, Han DK
Abstract
Sage-N's Sorcerer 2 provides an integrated data analysis system for comprehensive protein identification and characterization. It runs on a proprietary version of SEQUEST(R), the most widely used search engine for identifying proteins in complex mixtures. The protocol presented here describes the basic steps performed to process mass spectrometric data with Sorcerer 2 and how to analyze results using TPP and Scaffold. The unit also provides an overview of the SEQUEST(R) algorithm, along with Sorcerer-SEQUEST(R) enhancements, and a discussion of data filtering methods, important considerations in data interpretation, and additional resources that can be of assistance to users running Sorcerer and interpreting SEQUEST(R) results.
PMID: 19957274 [PubMed - indexed for MEDLINE]

Source: Current Protocols in Bioinformatics - November 12, 2014 Category: Bioinformatics Tags: Curr Protoc Bioinformatics Source Type: research