Next generation sequence assembly with AMOS.

Next generation sequence assembly with AMOS. Curr Protoc Bioinformatics. 2011 Mar;Chapter 11:Unit 11.8 Authors: Treangen TJ, Sommer DD, Angly FE, Koren S, Pop M Abstract A Modular Open-Source Assembler (AMOS) was designed to offer a modular approach to genome assembly. AMOS includes a wide range of tools for assembly, including the lightweight de novo assemblers Minimus and Minimo, and Bambus 2, a robust scaffolder able to handle metagenomic and polymorphic data. This protocol describes how to configure and use AMOS for the assembly of Next Generation sequence data. Additionally, we provide three tutorial examples that include bacterial, viral, and metagenomic datasets with specific tips for improving assembly quality. PMID: 21400694 [PubMed - indexed for MEDLINE]

http://www.ncbi.nlm.nih.gov/pubmed/21400694?dopt=Abstract

Source: Current Protocols in Bioinformatics - November 12, 2014 Category: Bioinformatics Tags: Curr Protoc Bioinformatics Source Type: research

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