General requirements for protein secretion by the F-like conjugation system R1

This study aims to understand mechanisms leading to the initiation of type IV secretion using conjugative plasmid paradigm R1. We analyze the general requirements for plasmid encoded conjugation proteins and DNA sequence within the origin of transfer (oriT) for protein secretion activity using a Cre recombinase reporter system. We find that similar to conjugative plasmid DNA strand transfer, activation of the R1 system for protein secretion depends on binding interactions between the multimeric, ATP-binding coupling protein and the R1 relaxosome including an intact oriT. Evidence for DNA independent protein secretion was not found. Highlights ► Mechanism of protein transfer by plasmid R1 conjugative T4 system requires relaxosome. ► Protein translocation depends on concomitant DNA transfer. ► Required functions of T4 coupling protein correlate for protein and DNA transfer.
Source: Plasmid - Category: Biotechnology Source Type: research