Subunit Composition of Ribosome in the < b > < i > yqgF < /i > < /b > Mutant Is Deficient in pre-16S rRNA Processing of < b > < i > Escherichia coli < /i > < /b >

Escherichia coli 16S, 23S, and 5S ribosomal RNAs (rRNAs) are transcribed as a single primary transcript, which is subsequently processed into mature rRNAs by several RNases. Three RNases (RNase III, RNase E, and RNase G) were reported to function in processing the 5 ′-leader of precursor 16S rRNA (pre-16S rRNA). Previously, we showed that a novel essential YqgF is involved in that processing. Here we investigated the ribosome subunits of theyqgFts mutant by LC-MS/MS. The mutant ribosome had decreased copy numbers of ribosome protein S1, suggesting that theyqgF gene enables incorporation of ribosomal protein S1 into ribosome by processing of the 5 ′-end of pre-16S rRNA. The ribosome protein S1 is essential for translation inE. coli; therefore, our results suggest that YqgF converts the inactive form of newly synthesized ribosome into the active form at the final step of ribosome assembly.J Mol Microbiol Biotechnol 2018;28:179 –182
Source: Journal of Molecular Microbiology and Biotechnology - Category: Microbiology Source Type: research