Identification of the full 26 cytochrome P450 (CYP) genes and analysis of their expression in response to benzo[α]pyrene in the marine rotifer Brachionus rotundiformis

Publication date: Available online 4 December 2018Source: Comparative Biochemistry and Physiology Part D: Genomics and ProteomicsAuthor(s): Jeonghoon Han, Jun Chul Park, Atsushi Hagiwara, Heum Gi Park, Jae-Seong LeeAbstractCytochrome P450s (CYPs) are a large gene superfamily that are found in all living organisms. CYPs have a key role in detoxification of xenobiotics and endogenous chemicals. Although aquatic invertebrate CYPs and their detoxification mechanisms have been reported, little is known about interspecific comparison of CYPs and their detoxification mechanism in the rotifer Brachionus spp. The aim of this study was to identify the entire CYPs in the rotifer Brachionus rotundiformis (B. rotundiformis) and compare B. rotundiformis-CYPs to the previously reported CYPs in other model Brachionus spp. (B. koreanus, B. plicatilis, and B. calyciflorus). To validate the model, the rotifer, specifically Brachionus rotundiformis was exposed various concentrations of B[α]P, which is widely used PAH xenobiotic, and analyzed gene expression in response to B[α]P. Here, in silico analysis results showed the total of 26 CYPs from the rotifer B. rotundiformis. Based on the phylogenetic analysis, the 26 B. rotundiformis-CYPs were separated into five different clans: 2, 3, 4, mitochondrial, and 46 clans in comparison to three rotifers species, B. koreanus, B. plicatilis, and B. calyciflorus. To understand the detoxification mechanisms of 26 B. rotundiformis-CYPs, we investigated tra...
Source: Comparative Biochemistry and Physiology Part D: Genomics and Proteomics - Category: Genetics & Stem Cells Source Type: research