B-RafV600E inhibits sodium iodide symporter expression via regulation of DNA methyltransferase 1.

In this study, we found that B-RafV600E inhibited NIS expression by the upregulation of its promoter methylation, and that specific regions of CpG islands of NIS promoter in B-RafV600E harboring PTC were highly methylated compared with surrounding normal tissue. Although DNA methyltransferase 3a and 3b (DNMT3a,3b) were not increased by B-RafV600E, DNMT1 expression was markedly upregulated in PTC and B-RafV600E expressing thyrocytes. Furthermore, DNMT1 expression was upregulated by B-RafV600E induced NF-κB activation. These results led us to conclude that NIS promoter methylation, which was induced by B-RafV600E, is one of the possible mechanisms involved in NIS downregulation in PTC. PMID: 25378232 [PubMed - in process]

http://www.ncbi.nlm.nih.gov/pubmed/25378232?dopt=Abstract

Source: exp Mol Med - November 9, 2014 Category: Molecular Biology Authors: Choi YW, Kim HJ, Kim YH, Park SH, Chwae YJ, Lee J, Soh EY, Kim JH, Park TJ Tags: Exp Mol Med Source Type: research