Vacuolating cytotoxin A (vacA) and cytotoxin ‐associated gene A (cagA) genotypes of resistant Helicobacter pylori strains isolated from raw and pasteurized milk

Foods with animal origins play a considerable role in transmission ofHelicobacterpylori. The current study was done to evaluate the genotyping patterns and antibiotic resistance properties ofH. pylori strains isolated from raw and pasteurized milk. Three hundred and fifty raw and pasteurized milk samples were collected. Samples were cultured on Wilkins Chalgren media. Antibiotic resistance pattern and genotyping were done using the disk diffusion and polymerase chain reaction, respectively. Sixty ‐eight out of 350 milk samples (19.42%) were positive forH. pylori. Prevalence ofH. pylori in raw and pasteurized milk samples were 44.00% and 1.00%, respectively.Helicobacterpylori strains exhibited the maximum prevalence of resistance against tetracycline (94.11%), ampicillin (91.17%), amoxicillin (88.23%), and erythromycin (69.11%).VacA s1a (98.52%),m1a (88.23%),m2 (79.41%), ands2 (77.94%) and alsovacA s1am1a (61.76%),s1am2 (51.94%),s2m2 (51.94%), ands2m1a (50.00%) were the most frequently detected genotypes.VacA s2/cagA+ (50.00%), s1a/cagA+ (47.05%), m1a/cagA+ (41.17%), and m2/cagA+ (33.82%) were the most frequently detected combined genotyping patterns. Boost prevalence ofH. pylori in raw and pasteurized milk exhibits that they may be the natural reservoirs of bacteria. SimilarvacA andcagA genotyping patterns ofH. pylori strains recommends that they have similar routes of infection.Practical applicationsDue to the high consumption rate of raw and pasteurized milk samples, they...
Source: Journal of Food Safety - Category: Food Science Authors: Tags: ORIGINAL ARTICLE Source Type: research