Antimicrobial resistance and genotyping of vacA, cagA, and iceA alleles of the Helicobacter pylori strains isolated from traditional dairy products

AbstractDairy products play a considerable role in transmission ofHelicobacter pylori. The current study was done to assess phenotypic characters of antimicrobial resistance and genotyping pattern ofvacA,cagA, andiceA alleles among theH. pylori strains isolated from dairy products. Eight ‐hundred dairy samples were collected and cultured. Antimicrobial resistance and genotyping patterns were assessed using disk diffusion and polymerase chain reaction, respectively. Thirty‐one out of 800 (3.87%) traditional dairy product samples were positive forH. pylori. Cheese (11.53%) samples had the highest incidence ofH. pylori, while yoghurt (1.11%) had the lowest.Helicobacter pylori strains harbored the highest incidence of resistance against ampicillin (93.54%), amoxicillin (93.54%), tetracycline (90.32%), erythromycin (80.64%), and metronidazole (77.41%).VacA s1a (90.32%),m1a (90.32%),s2 (80.64%) andm2 (77.19%),cagA (51.61%), andiceA1 (32.25%) were the most routinely identified genotypes.S1am1a (67.74%),s2m1a (64.51%), ands1am2 (51.61%) were the most routinely identified genotyping pattern. Simultaneous presence ofvacA,cagA, andiceA genotypes in antimicrobial resistantH. pylori strains indicates important public health issue regarding the consumption of dairy products. However, additional researches are required to find molecular genetic homology and other epidemiological aspects ofH. pylori in dairy products.Practical applicationsDue to the high consumption rate of dairy samples...
Source: Journal of Food Safety - Category: Food Science Authors: Tags: ORIGINAL ARTICLE Source Type: research