Analysis of Bacillus cereus cell viability, sublethal injury, and death induced by mild thermal treatment

AbstractAs the most cost ‐effective tool to ensure microbial safety, thermal processing induces a large portion of sublethal injury presenting a potential hazard to food safety. Thermal treatment at 63 °C for 1 min injured 2.22 log cfu/ml totally, half of which were sublethally injured using plate counting method. Afte r 2 min, the inactivation log ofBacillus cereus reached 1.55 while the sublethally injured log even reached 2.16. As for the sublethally injured rate of theB. cereus, it was over 65% after 0.5 min and kept ever ‐increase with time. In the end, the injured rate arrived at 99.30% after 2 min processing time. Partial esterase enzyme inactivation was found after 0.5 min heat treatment with flow cytometry combining carboxyfluorescein diacetate/propidium iodide (PI) double‐staining, butB. cereus was not dyed by PI at 63  °C. Comparing with the initial protein concentration of 0.57 ± 0.02 μg/ml, the leakage of the protein was not so notable though the general trend was increasing with duration of heat. Scanning and transmission electron microscopy analysis revealed that a portion of cells morphology and s tructure had changed after thermal process.Practical applicationsBacillus cereus is an endospore forming pathogenic, causing foodborne illnesses and outbreaks all over the world. This research indicated that thermal treatment at 63  °C was a sublethal stress forB. cereus that a portion of cells were sublethally injured which could resume growth...
Source: Journal of Food Safety - Category: Food Science Authors: Tags: ORIGINAL ARTICLE Source Type: research