Aflatoxin G1 induced TNF ‐α‐dependent lung inflammation to enhance DNA damage in alveolar epithelial cells

In this study, we found increased DNA damage and cytochrome P450 (CYP2A13) expression in AFG1‐induced inflamed lung tissues. Furthermore, we treated the mice with a soluble tumor necrosis factor (TNF)‐α receptor and AFG1 and found that TNF ‐α neutralization inhibited the AFG1‐induced chronic lung inflammation in vivo, and then reversed the CYP2A13 expression and DNA damage in AT‐II cells. The results suggest that AFG1 induces TNF ‐α‐dependent lung inflammation to regulate 2A13 expression and enhance DNA damage in AT‐II cells. Then, we treated the primary mice AT‐II cells and human AT‐II like cells (A549) with AFG1 and TNF ‐α and found that TNF‐α enhanced the AFG1‐induced DNA damage in mice AT‐II cells as well as A549 cells in vitro. In AFG1‐exposed A549 cells, TNF‐α‐enhanced DNA damage and apoptosis were reversed by CYP2A13 small interfering RNA. Blocking NF‐κB pathway inhibited the TNF‐α‐enhanced CYP2A13 upregulation and DNA damage confirming that the CYP2A13 upregulation by TNF‐α plays an essential role in the acti vation of AFG1 under inflammatory conditions. Taken together, our findings suggest that AFG1 induces TNF ‐α‐dependent lung inflammation, which upregulates CYP2A13 to promote the metabolic activation of AFG1 and enhance oxidative DNA damage in AT ‐II cells.

Source: Journal of Cellular Physiology - November 27, 2018 Category: Cytology Authors: Peilu Shao, Ningfei Guo, Can Wang, Mei Zhao, Li Yi, Chunping Liu, Lifei Kang, Lei Cao, Ping Lv, Lingxiao Xing, Xianghong Zhang, Haitao Shen Tags: ORIGINAL RESEARCH ARTICLE Source Type: research