Effective attenuation of glyphosate ‐induced oxidative stress and granulosa cell apoptosis by vitamins C and E in caprines

The present study aims at assessing the GLP (0.1, 2.0, and 4.0  mg/ml) induced granulosa cells toxicity and evaluating the mitigating effects of vitamins C& E (0.5  mM and 1.0 mM) in healthy caprine antral follicles, cultured in vitro in dose and time (24, 48, and 72 hr) dependent manner and subjected to various cytotoxic and geno‐toxic analysis namely classic histology, EB/AO differential staining, oxidative stress parameters, and antioxidant enzymati c activity. Pesticides are known to cause a wide range of reproductive problems that possess degenerative effects on mammalian fertility. Glyphosate (GLP), a broad ‐spectrum organophosphate herbicide, is known to be a potent mammalian toxicant. The present study aims at assessing the GLP‐induced (0.1, 2.0, and 4.0 mg/ml) granulosa cells toxicity and evaluating the mitigating effects of vitamins C and E (0.5 mM and 1.0 mM) in healthy caprine antral fo llicles, cultured in vitro in a dose‐ and time‐dependent manner (24, 48, and 72 hr) and subjected to various cytotoxic and geno‐toxic analysis, namely, classic histology, EB/AO differential staining, oxidative stress parameters, and antioxidant enzymatic activity. The histomorphological anal ysis and EB/AO staining elucidated increase in the incidence of apoptotic attributes within granulosa cells with increasing dose and duration of the GLP treatment. The highest apoptotic frequency was observed at 4.0 mg/ml GLP after 72‐hr exposure duration in comp...
Source: Molecular Reproduction and Development - Category: Reproduction Medicine Authors: Tags: RESEARCH ARTICLE Source Type: research