Inhibitory effect of ethyl acetate fraction of Solanum macrocarpon L. leaves on cholinergic, monoaminergic, and purinergic enzyme activities

This study evaluated the inhibitory effect of ethyl acetate fraction ofSolanum macrocarponL. leaves on cholinergic, monoaminergic, purinergic enzyme activities and identification of bioactive compounds. 2,2 ‐azino‐bis‐3‐ethylbenthiazoline‐6‐sulphonic acid (ABTS), metal ion chelating radicals scavenging activity, total phenolic and flavonoid contents were determined using standard methods. The inhibitory effects of the ethyl acetate fraction ofS. macrocarpon on the specified enzymes were determined spectroscopically. Ethyl acetate fraction ofS. macrocarpon showed a high ABTS radical scavenging activity and metal ion chelating radical scavenging activity. The fraction showed high contents of phenol and flavonoid. The fraction also inhibited AChE, BChE, MOA, tyrosinase, arginase, ecto ‐5′ ‐nucleotidase, PDE‐5, ACE and stimulated the activity of Na+/K+‐ATPase. Bioactive compounds present in ethyl acetate fraction ofS. macrocarponleaves include kaempferol ‐3‐rutinoside, rutin among others. Ethyl acetate fraction ofS. macrocarponleaves inhibited cholinergic, monoaminergic, and purinergic enzymes and had a stimulatory effect on Na+/K+‐ATPase.Practical applicationsSolanum macrocarpon L. leaves are commonly consumed as vegetable. The enormous medicinal potential of the ethyl acetate fraction is invaluable as it ’s a probable source of polyphenols characterized with strong antioxidant activity. In addition, the leaves are used as functional foods and may p...
Source: Journal of Food Biochemistry - Category: Food Science Authors: Tags: FULL ARTICLE Source Type: research