Synthesis, characterization and biological applications of some substituted pyrazoline based palladium (II) compounds

Synthesis and characterization of pyrazoline ‐based Pd (II) complexes have been carried out as an alternative to platinum‐based drugs. The complexes have been evaluated for diverse biological activities like DNA binding, nucleolytic, antibacterial and antituberculosis activities and cytotoxicity with appropriate methods along with kinetic and thermodynamic parameters. Palladium (II) complexes of the type [PdLCl2] (where L  = substituted pyrazoline ligands) have been synthesized. The metal complexes (5a‐5f) have been characterized by various spectroscopic and analytical techniques like1H ‐NMR,13C ‐NMR, LC–MS, IR, Energy‐dispersive X‐ray (EDX), electronic spectroscopy, thermogravimetric analysis (TGA), elemental analysis and conductance measurement. Complexes to herring sperm DNA (HS‐DNA) binding has been explored by absorption titration and binding constant (Kb) as well as Gibb's free energy have been evaluated. Complex 5b shows the highest binding constant value. So, thermodynamic parameters of 5b ‐HS DNA complex at different temperature have been evaluated. Also, Viscosity measurement and molecular modeling studies have been performed to know the binding mode of complexes. Based on the observations, an intercalative binding mode of DNA has been proposed. Further confirmation of intercalati ve mode of DNA binding has been taken by fluorescence spectroscopy and results are in a good agreement with absorption titration, viscosity measurement and molecular ...
Source: Applied Organometallic Chemistry - Category: Chemistry Authors: Tags: FULL PAPER Source Type: research