Cu (II) tyrosinate complexes containing methyl substituted phenanthrolines: Synthesis, X ‐ray crystal structures, biomolecular interactions, antioxidant activity, ROS generation and cytotoxicity

A new ternary copper (II) tyrosinate complexes containing methyl substituted 1,10 ‐phenanthrolines have been synthesized and characterized by CHN analysis, cyclic voltammetry, ESI‐MS, FTIR and single‐crystal X‐ray diffraction techniques. They have been tested for theirin vitro DNA/BSA interactions by the spectroscopic methods. These complexes exhibited higher cytotoxic and ROS activities. In this paper, three new copper (II) complexes, [Cu(4 ‐mphen)(tyr)(H2O)]ClO4(1), [Cu(5 ‐mphen)(tyr)(H2O)]ClO4·1.5H2O(2) and [Cu (tmphen)(tyr)(NO3)]0.5H2O(3) (4 ‐mphen: 4‐methyl‐1,10‐phenanthroline, 5‐mphen: 5‐methyl‐1,10‐phenanthroline, tmphen: 3,4,7,8‐tetramethyl‐1,10‐phenanthroline and tyr: L‐tyrosine), were synthesized and characterized using elemental analyses, FT‐IR, ESI‐MS, cyclic voltammetry and single‐crystal X‐ray d iffraction. It was found that the complexes adopt a distorted five‐coordinate square pyramidal geometry. The interaction of the three complexes with calf thymus DNA was also investigated using UV–visible absorption spectra, ethidium bromide and Hoechst 33258 displacement assay and thermal denatu ration. The DNA cleavage activity of the complexes, monitored using gel electrophoresis, showed significant damage of the pUC19 plasmid DNA. Binding activity of bovine serum albumin (BSA) reveals that these complexes can strongly quench the fluorescence of BSA through a static quenching mechanism. T he results suggested that intera...
Source: Applied Organometallic Chemistry - Category: Chemistry Authors: Tags: FULL PAPER Source Type: research