Novel peptide dermaseptin ‐PS1 exhibits anticancer activity via induction of intrinsic apoptosis signalling

In this study, a novel AMP named Dermaseptin ‐PS1 was isolated and identified fromPhyllomedusa sauvagei, it showed strong antimicrobial activities against three types of microorganisms. In vitro antiproliferative studies on human glioblastoma U ‐251 MG cells indicated that Dermaseptin‐PS1 disrupted cell membranes at the concentrations of 10−5 M and above, while the cell membrane integrity was not affected when concentrations were decreased to 10−6 M or lower. Further examinations revealed that, at the relatively low concentration (10−6 M), Dermaseptin‐PS1 induced apoptosis through mitochondrial‐related signal pathway in U‐251 MG cells. Thus, for the first time, we report a novel frog skin derived AMP with anticancer property by distinct mechanisms, which largely depends on its concentration. Together, our study provides ne w insights into the mechanism‐illustrated drug design and the optimisation of dose control for cancer treatment in clinic.

Source: Journal of Cellular and Molecular Medicine - November 20, 2018 Category: Molecular Biology Authors: Qilin Long, Lei Li, Hao Wang, Miaoran Li, Lei Wang, Mei Zhou, Qiaozhu Su, Tianbao Chen, Yuxin Wu Tags: ORIGINAL ARTICLE Source Type: research