Molecular genetic investigation of dental attrition in mice

Publication date: April 2013 Source:Pediatric Dental Journal, Volume 23, Issue 1 Author(s): Chiaki Tatsumi , Kei Okamoto Inbred C3H/HeJSlc (C3H) and C57BL/6JSlc (B6) strains of mice fed a powdered high-cholesterol diet for 240 days were reported previously to exhibit high and low susceptibility to dental attrition, respectively. Although a number of physical and physiological factors are known to be related to attrition, genetic factors have rarely been discussed. In the present study, F2 mice were used to investigate the loci of genes that may be involved in attrition. The F1 strain was acquired by cross-breeding the C3H and B6 inbred strains, and the F2 strain was generated by intercrossing the F1 mice. The attrition state of the 96 F2 mice that were acquired was examined using a stereoscopic microscope. The ratio of attrition area was calculated and scored, and then the F2 mice were separated into high-attrition and low-attrition groups. The top 10-ranked F2 mice for ratio of attrition area were designated high-attrition susceptibility mice, and the lowest 10-ranked F2 mice for ratio of attrition area were designated low-attrition susceptibility mice. Using DNA markers with polymorphisms in the C3H and B6 strains, linkage analysis and quantitative trait locus analysis were conducted. The results revealed significant values for markers D11Mit301, D15Mit261, and D15Mit171 on mouse chromosomes 11 and 15, respectively and a suggestive value for D5Mit139 on chromosome 5, ...
Source: Pediatric Dental Journal - Category: Dentistry Source Type: research