Transcriptome analyses of the ginseng root rot pathogens Cylindrocarpon destructans and Fusarium solani to identify radicicol resistance mechanisms

Publication date: Available online 23 November 2018Source: Journal of Ginseng ResearchAuthor(s): Taiying Li, Jin-Hyun Kim, Boknam Jung, Sungyeon Ji, Mun Won Seo, You Kyoung Han, Sung Woo Lee, Yeoung Seuk Bae, Hong-Gyu Choi, Seung-Ho Lee, Jungkwan LeeAbstractBackgroundThe ascomycete fungi Cylindrocarpon destructans (Cd) and Fusarium solani (Fs) cause ginseng root rot and significantly reduce the quality and yield of ginseng. Cd produces the secondary metabolite radicicol, which targets the molecular chaperone Hsp90. Fs is resistant to radicicol, whereas other fungal genera associated with ginseng disease are sensitive to it. Radicicol resistance mechanisms have not yet been elucidated.MethodsTranscriptome analyses of Fs and Cd mycelia treated with or without radicicol were conducted using RNA-seq. All of the differentially expressed genes (DEGs) were functionally annotated using the Fusarium graminearum transcript database. Additionally, deletions of two transporter genes identified by RNA-seq were created to confirm their contributions to radicicol resistance.Results and conclusionTreatment with radicicol resulted in upregulation of chitin synthase and cell wall integrity genes in Fs and upregulation of NADH dehydrogenase and sugar transporter genes in Cd. Genes encoding an ABC transporter, an aflatoxin efflux pump, ammonium permease 1 (mep1), and nitrilase were differentially expressed in both Fs and Cd. Among these four genes, only the ABC transporter was upregulated in bot...
Source: Journal of Ginseng Research - Category: Complementary Medicine Source Type: research