Roles of miRNA ‐1 and miRNA‐133 in the proliferation and differentiation of myoblasts in duck skeletal muscle

MicroRNA (miRNA) ‐1 targets histone deacetylase 4 to promote the differentiation of duck myoblasts and miRNA‐133 may affect serum response factor and transforming growth factor β receptor 1 expression to promote proliferation, which indicates that miRNA‐1 and miRNA‐133 play different important roles in skel etal muscle development. AbstractMicroRNA (miRNA) ‐1 and miRNA‐133 are derived from the same bicistronic pairs with roles in skeletal muscle development. Many investigations have focused on the role of miRNA‐1 and miRNA‐133 in the regulation of skeletal muscle development in mammals and fish. However, the mechanisms of miRNA‐1 and miRNA‐ 133 underlying the differences in skeletal muscle development between different breeds are not well known. Our study found that the weights of body and breast at 42 days of age were greater in Cherry Valley ducks than in Putian ducks and the areas of breast muscle fibers increased with age; the area s of muscle fibers of Cherry Valley ducks were always greater than those of Putian ducks. Besides, quantitative reverse‐transcriptase polymerase chain reaction analysis revealed that relatively high levels of miRNA‐1 and miRNA‐133 were detected in heart, breast, and leg muscles compared with t he liver, spleen, lung, kidney, and the expression levels of miRNA‐1 and miRNA‐133 remained stable in the embryo stage, and in the growth period, the fluctuation in miRNA expression levels in Putian ducks was considerably hi...
Source: Journal of Cellular Physiology - Category: Cytology Authors: Tags: ORIGINAL RESEARCH ARTICLE Source Type: research