Effect of point sampling density in quantifying mouse lung emphysema

In this study, we examined this question by focusing on some of the relevant sampling levels in mice with extensive elastase‐induced emphysema. Using fixed samples of tissue blocks, we varied the number of sampling points or intercepts from about 25 to 1000 in control and emphysematous lungs. Our results show that, at the sampling levels investigated, even with the increased heterogeneity in the lung tissue damage caused by elastase, the number of sampling points needed to detect changes is similar to what is needed for control mice. Anat Rec, 2014. © 2014 Wiley Periodicals, Inc.

Source: The Anatomical Record Part B: The New Anatomist - November 1, 2014 Category: Anatomy Authors: Nathachit Limjunyawong, Alexandra Kearson, Sandhya Das, Wayne Mitzner Tags: Full Length Article Source Type: research