Application of stable isotopes to investigate the metabolism of fatty acids, glycerophospholipid and sphingolipid species

Publication date: April 2014 Source:Progress in Lipid Research, Volume 54 Author(s): Josef Ecker , Gerhard Liebisch Nature provides an enormous diversity of lipid molecules that originate from various pathways. To gain insight into the metabolism and dynamics of lipid species, the application of stable isotope-labeled tracers combined with mass spectrometric analysis represents a perfect tool. This review provides an overview of strategies to track fatty acid, glycerophospholipid, and sphingolipid metabolism. In particular, the selection of stable isotope-labeled precursors and their mass spectrometric analysis is discussed. Furthermore, examples of metabolic studies that were performed in cell culture, animal and clinical experiments are presented.

Source: Progress in Lipid Research - November 5, 2014 Category: Lipidology Source Type: research