Inflammatory molecules produced by meniscus and synovium in early and end ‐stage osteoarthritis: a coculture study

Inflammatory mediators, metalloproteinases, and metalloproteinases inhibitors are produced by meniscus and synovium from early and end ‐stage osteoarthritic patients. Production of inflammatory molecules by synovium and meniscus can trigger inflammatory signals in early osteoarthritis (OA) patients, and induce extracellular matrix loss in the progressive and final stages of OA pathology. The interaction between synovium and menis cus is suggested in early and late osteoarthritic patients and it may provide a basis for identifying the mechanisms contributing to the development of OA associated with meniscal tear. AbstractThe aim of this study was to identify the molecules and pathways involved in the cross ‐talk between meniscus and synovium that may play a critical role in osteoarthritis (OA) pathophysiology. Samples of synovium and meniscus were collected from patients with early and end‐stage OA and cultured alone or cocultured. Cytokines, chemokines, metalloproteases, and their inhibitors were evaluated at the gene and protein levels. The extracellular matrix (ECM) changes were also investigated. In early OA cultures, higher levels of interleukin‐6 (IL‐6) and IL‐8 messenger RNA were expressed by synovium and meniscus in coculture compared with meniscus cultured alone. RANTES releas e was significantly increased when the two tissues were cocultured compared with meniscus cultured alone. Increased levels of matrix metalloproteinase‐3 (MMP‐3) and MMP‐10 pro...

Source: Journal of Cellular Physiology - November 19, 2018 Category: Cytology Authors: Marta Favero, Elisa Belluzzi, Giovanni Trisolino, Mary B Goldring, Steven R Goldring, Augusto Cigolotti, Assunta Pozzuoli, Pietro Ruggieri, Roberta Ramonda, Brunella Grigolo, Leonardo Punzi, Eleonora Olivotto Tags: ORIGINAL RESEARCH ARTICLE Source Type: research