IL ‐17 and IL‐22 elicited by a DNA vaccine encoding ROP13 associated with protection against Toxoplasma gondii in BALB/c mice

AbstractToxoplasma gondii, an intracellular parasitic protozoan, is capable of infecting man and all warm ‐blooded animals. Cell‐mediated immunity is vital in mounting protective responses againstT. gondii infection. Recent studies have shown that T ‐helper (Th) 17 responses may play a key role in parasite control. In this current study, we constructed a DNA vaccine encodingT. gondii ROP13 in a pcDNA vector. Groups of BALB/c mice were immunized intramuscularly with pcROP13 or controls and challenged with the RH strain ofT. gondii. The results showed that immunization with pcROP13 could elicit an antibody response againstT. gondii. The expression of the canonical Th17 cytokines, interleukin (IL) ‐17 and IL‐22, were significantly increased after immunization with pcROP13 compared with control groups (p <  0.05). Furthermore, vaccination resulted in a significant decrease in parasite load (p <  0.05). The induction of Th17 related cytokines, using a ROP13 DNA vaccine, againstT. gondii should be considered as a potential vaccine approach for the control of toxoplasmosis.
Source: Journal of Cellular Physiology - Category: Cytology Authors: Tags: ORIGINAL RESEARCH ARTICLE Source Type: research