Prevalence, genotyping, serotyping, and antibiotic resistance of isolated Salmonella strains from industrial and local eggs in Iran

AbstractThis present study aimed to evaluate prevalence and characterization of serotypes, antimicrobial resistance (AMR) and genotypic profiles ofSalmonella isolates in industrial and local eggs from Zanjan province of Iran. A total of 120 egg samples were collected and processed according to bacteriological analytical manual to isolateSalmonella strains from both eggs surface and contents. PCR method and genotyping were used to verify, identification and classification of isolates by screeninginvA genes and 16  s‐rRNA gene sequencing.Salmonella contamination rate in eggshell and contents of industrial and local eggs were 0% and 1.66%, respectively. High degree of AMR was observed to nalidixic acid and erythromycin. Gene sequencing for each isolates showed more similarity withSalmonella enterica subsp.enterica serovar Enteritidis andSalmonella enterica subsp.enterica serovar Typhimurium strains.Practical applicationsSalmonella contamination in eggs and the role of healthy chickens as reservoir and distributer ofSalmonella in poultry farms can be a common food safety concern for public health. Therefore, Continuous monitoring of presence Salmonella and their antimicrobial resistance is of critical importance. Present study gives an insight of the current statues of egg's contamination withSalmonella isolates, their antimicrobial resistance, genotupic profile, and epidemiological relationship in Iran.
Source: Journal of Food Safety - Category: Food Science Authors: Tags: ORIGINAL ARTICLE Source Type: research