Histological and biochemical aspects of compatible and incompatible wheat- Puccinia striiformis interactions

Publication date: Available online 19 November 2018Source: Physiological and Molecular Plant PathologyAuthor(s): Samar.M. Esmail, Reda.I. Omara, Khaled.A.A. Abdelaal, Yaser.M. HafezAbstractThe resistant, slow and fast-rusting cultivars were selected from 15 wheat cultivars to study biochemical and histological aspects of compatible and incompatible wheat-Puccinia striiformis interactions. Final rust severity (%) and area under disease progress curve were significantly decreased in resistant cultivars compared with susceptible ones. Biochemical analysis revealed that reactive oxygen species (ROS), i.e. superoxide (O2•-) and hydrogen peroxide (H2O2) significantly accumulated in the susceptible cultivars. While, activities of catalase (CAT), peroxidase (POX) and chlorophyll concentrations were increased in resistant cultivars. Histological examination using scanning and transmission electron microscope indicated that approximately all haustoria and hyphae were inhibited in resistant cultivar (Misr-1), development of haustorial mother cells and haustoria were retarded, and they were abnormal. On the other hand, large amounts of intercellular hyphae presented in the intercellular spaces and many haustoria were produced in fast-rusting cultivar (Sids-12). Nevertheless, a little of sours and spores on upper epidermis and in mesophyll tissue were observed in slow-rusting cultivars.
Source: Physiological and Molecular Plant Pathology - Category: Molecular Biology Source Type: research