Long noncoding RNA STXBP5 ‐AS1 inhibits cell proliferation, migration, and invasion via preventing the PI3K/AKT against STXBP5 expression in non–small‐cell lung carcinoma

In our study, the functional role and mechanism of syntaxin ‐binding protein 5‐antisense RNA 1 (STXBP5‐AS1) in non–small‐cell lung carcinoma (NSCLC) have been explored in our study. Our results showed that STXBP5‐AS1 could inhibit cell proliferation, migration, and invasion abilities in NSCLC by preventing the phosphatidylinositol 3 kinase/protei n kinase B signaling pathway against STXBP5 expression. AbstractLong noncoding RNAs participate in carcinogenesis and tumor progression in non –small‐cell lung carcinoma (NSCLC), but the mechanisms underlying NSCLC tumorigenesis remain to be fully elucidated. Here, we reported the functional role and potential mechanism of long noncoding RNA syntaxin‐binding protein 5‐antisense RNA 1 (STXBP5‐AS1) in NSCLC. First, our data reveale d that the expression levels of STXBP5‐AS1 in 31 NSCLC tissues were lower than in adjacent tissues using quantitative polymerase chain reaction (qPCR) and its expression was significantly associated with tumor metastasis of NSCLC patients. Moreover, CCK‐8, scratch wound healing and transwell ass ay suggested that upregulation of STXBP5‐AS1 repressed the proliferation, migration, and invasion in A549, NCI‐H292, and NCI‐H460 cells. To explore the potential mechanism of STXBP5‐AS1 in NSCLC, we first investigated the relationship among STXBP5‐AS1, STXBP5, and AKT1 in A549 cells. Resul ts indicated that STXBP5‐AS1 was negatively related with STXBP5 and AKT1 at messenger RNA ...
Source: Journal of Cellular Biochemistry - Category: Biochemistry Authors: Tags: RESEARCH ARTICLE Source Type: research