Evaluation of antibacterial activity of hexanedioic acid isolated from Hermetia illucens larvae

This study was carried out to evaluate the effect of a new substance causing antibacterial activity against various pathogenic bacteria, including methicillin-resistant Staphylococcus aureus (MRSA). The antibacterial activity of hexanedioic acid was determined using different antimicrobial indicators such as turbidometric assay, resazurin assay, and agar disk diffusion. Hexanedioic acid showed the selective-growth inhibitory effects against the growth and proliferation of Staphylococcus aureus, MRSA, Klebsiella pneumonia, and Shigella dysenteriae in a concentration dependent manner. The growth inhibitory zones of bacteria treated with 80μg/ml of hexanedioic acid for 24h were measured as 18.27±0.18, 23.35±0.15, 16.62±0.18, and 12.96±0.24mm, respectively. The minimum inhibitory concentration (MIC) values of hexanedioic acid against the viability of these bacteria for 24h were measured as 140.377, 137.369, 139.117, and 139.704μg/ml, respectively. These results demonstrate that hexanedioic acid has antibacterial properties that effectively inhibit the growth/proliferation of pathogenic bacteria. Furthermore, this study suggests novel aspects for the utilization of hexanedioic acid as a medicinal substance.
Source: Journal of Applied Biomedicine - Category: Biotechnology Source Type: research