Identification of sperm motility markers in bovine transition protein genes
ContentsTransition proteins (TNPs) are essential in chromatin condensation during spermiogenesis, and hence, they are the candidate genes for identifying sperm motility markers. Coding and in silico predicted promoter regions of these genes were investigated in crossbred and purebred cattle, and also, their mRNA quantification was done to explore its use as a diagnostic tool of infertility. PCR âSSCP analysis revealed two band patterns in fragment III ofTNP1 and fragment II ofTNP2 gene. Sequence analysis revealed a deletion of âGâ nucleotide in 3â˛UTR region ofTNP1 and C>T SNP in intronic region ofTNP2 gene. Least square analysis of variance did not reveal any significant influence of nucleotide deletion on any sperm motility parameters in both crossbred and purebred cattle. However, C>T SNP had a significant effect on initial progressive motility (p <  0.05) in purebred cattle and postâthaw motility in overall cattle population. RTâqPCR analysis did not reveal any significant variation inTNP1 andTNP2 gene expression among poorly motile and good quality spermatozoa of Vrindavani bulls.
Source: Reproduction in Domestic Animals - Category: Reproduction Medicine Authors: Yathish Hirenallur Maheshwarappa,
Subodh Kumar,
Rajni Chaudhary,
Chinmoy Mishra,
Sivakumar Ayyar,
Amit Kumar,
Anuj Chauhan,
Subrata Kumar Ghosh,
Manjit Panigrahi,
Abhijit Mitra Tags: ORIGINAL ARTICLE Source Type: research