Identification of sperm motility markers in bovine transition protein genes

ContentsTransition proteins (TNPs) are essential in chromatin condensation during spermiogenesis, and hence, they are the candidate genes for identifying sperm motility markers. Coding and in silico predicted promoter regions of these genes were investigated in crossbred and purebred cattle, and also, their mRNA quantification was done to explore its use as a diagnostic tool of infertility. PCR ‐SSCP analysis revealed two band patterns in fragment III ofTNP1 and fragment II ofTNP2 gene. Sequence analysis revealed a deletion of “G” nucleotide in 3′UTR region ofTNP1 and C>T SNP in intronic region ofTNP2 gene. Least square analysis of variance did not reveal any significant influence of nucleotide deletion on any sperm motility parameters in both crossbred and purebred cattle. However, C>T SNP had a significant effect on initial progressive motility (p <  0.05) in purebred cattle and post‐thaw motility in overall cattle population. RT‐qPCR analysis did not reveal any significant variation inTNP1 andTNP2 gene expression among poorly motile and good quality spermatozoa of Vrindavani bulls.
Source: Reproduction in Domestic Animals - Category: Reproduction Medicine Authors: Tags: ORIGINAL ARTICLE Source Type: research