Longitudinal monitoring of anti ‐saliva antibodies as markers of repellent efficacy against Phlebotomus perniciosus and Phlebotomus papatasi in dogs

Dogs naturally exposed toLeishmania vectors, the sandfliesPhlebotomus perniciosus andPhlebotomus papatasi, and treated preventively with sandfly repellents developed antibodies against the insects' salivary proteins. Anti ‐P. perniciosus and ‐P. papatasi saliva antibody concentrations were low, which suggests the repellent limited sandfly bites and is useful in canine leishmaniasis control. Longitudinal monitoring of anti ‐saliva antibodies is a good method of marking insect repellent efficacy againstP. perniciosus andP. papatasi in dogs. AbstractA 2 ‐year longitudinal study of enzyme‐linked immunosorbent assay (ELISA) antibodies againstPhlebotomus perniciosus andPhlebotomus papatasi (Diptera: Psychodidae) sandfly saliva was performed in 32 Beagle dogs treated preventively with an imidacloprid –permethrin topical insecticide in an endemic area in Spain. Dogs were grouped into three sandfly exposure groups according to the time of inclusion in the study. Assays analysed immunoglobulin G (IgG) against salivary gland homogenates (SGH) of both species and recombinantP. papatasi rSP32 andP. perniciosus rSP03B proteins in serum. The dogs were participating in aLeishmania infantum (Kinetoplastida: Trypanosomatidae) vaccine trial and were experimentally infected with the parasite in the second year. No dog acquired naturalL. infantum infections during the first year, but most developed anti ‐saliva antibodies, and median log‐transformed optical densities (LODs) were s...
Source: Medical and Veterinary Entomology - Category: Veterinary Research Authors: Tags: Original Article Source Type: research