GATA2 zinc finger 2 mutation found in acute myeloid leukemia impairs myeloid differentiation

Publication date: 2013 Source:Leukemia Research Reports, Volume 2, Issue 1 Author(s): Keiko Niimi , Hitoshi Kiyoi , Yuichi Ishikawa , Fumihiko Hayakawa , Shingo Kurahashi , Rika Kihara , Akihiro Tomita , Tomoki Naoe We identified two novel GATA2 mutations in acute myeloid leukemia (AML). One mutation (p.R308P-GATA2) was a R308P substitution within the zinc finger (ZF)-1 domain, and the other (p.A350_N351ins8-GATA2) was an eight-amino-acid insertion between A350 and N351 residues within the ZF-2 domain. p.R308P-GATA2 did not affect DNA-binding and transcriptional activities, while p.A350_N351ins8-GATA2 reduced them, and impaired G-CSF-induced granulocytic differentiation of 32D cells. Although p.A350_N351ins8-GATA2 did not show a dominant-negative effect over wild-type (Wt)–GATA2 by the reporter assay, it might be involved in the pathophysiology of AML by impairing myeloid differentiation because of little Wt-GATA2 expression in primary AML cells harboring the p.A350_N351ins8 mutation.
Source: Leukemia Research Reports - Category: Hematology Source Type: research