MiR ‐130b promotes the progression of oesophageal squamous cell carcinoma by targeting SASH1

AbstractMiR ‐130b and SAM and SH3 domain containing 1 (SASH1) play an important role in many types of human cancers. The aim of our research was to study their interactions in the process of the proliferation and aggressiveness of oesophageal squamous cell carcinoma (ESCC) cells. Microarray analysis was done to screen the differentially expressed genes in the ESCC tissues. miR ‐130b andSASH1mRNA levels in the ESCC tissues and cells were detected by qRT ‐PCR. Dual luciferase reporter system was used to verify the target relationship between miR‐130b andSASH1. The effects of miR ‐130b onSASH1 expression were explored by western blot in KYSE30 and TE1 cell lines. CCK ‐8 assay, flow cytometry, Transwell, and wound healing assays were conducted to explore the effects of miR‐130b andSASH1 in  vitro. In addition, in vivo experiments were conducted to study the roles of miR‐130b andSASH1. miR ‐130b was highly expressed, whileSASH1 was the opposite in both the ESCC tissues and cells. The expression ofSASH1 was inhibited by the direct binding of miR ‐130b. The inhibition of miR‐130b reduced the proliferation and aggressiveness of ESCC cells, while it also induced apoptosis and cell cycle arrest in the ESCC cells by suppressingSASH1. The in  vivo assay suggested that the overexpression of miR‐130b promoted the growth of ESCC tumours. MiR‐130b was up‐regulated in the ESCC tumour tissues and cells, acting as a tumour promoter. A stimulating effect was demons...
Source: Journal of Cellular and Molecular Medicine - Category: Molecular Biology Authors: Tags: ORIGINAL ARTICLE Source Type: research