Broadly reactive real ‐time reverse transcription‐polymerase chain reaction assay for the detection of human sapovirus genotypes

In this study, we report the development of a broader‐range sapovirus real‐time RT‐PCR assay. The assay can detect 2.5 × 107 and 2.5  × 101 copies of sapovirus  and therefore is as sensitive as the previous test. Analysis using clinical stool specimens or synthetic DNA revealed that the new system detected strains representative of all the 18 human sapovirus genotypes: GI.1‐7, GII.1‐8, GIV.1, and GV.1, 2. No cross‐reactivity was observed against oth er representative common enteric viruses (norovirus, rotavirus, astrovirus, and adenovirus). This new assay will be useful as an improved, broadly reactive, and specific screening tool for human sapoviruses.
Source: Journal of Medical Virology - Category: Virology Authors: Tags: RESEARCH ARTICLE Source Type: research