Detection of Newly Synthesized Proteins via Metabolic Incorporation of Non-natural Amino Acid

This study provided a method for the detection of a specific newly synthesized protein, and characterization of specific protein turnover.Graphical abstractThe TNF-α produced by LPS stimulating cells was labeled with biotin tags via metabolic incorporation of non-natural amino acid and click chemistry which was then specifically captured by the antibody adsorbed on the solid support and detected.
Source: Chinese Journal of Analytical Chemistry - Category: Chemistry Source Type: research