microRNA ‐383 regulates cell viability and apoptosis by mediating Wnt/β‐catenin signaling pathway in non–small cell lung cancer

AbstractThe aim of this study was to investigate the roles of microRNA ‐383 (miRNA‐383) in progression of non–small cell lung cancer (NSCLC) and the potential mechanism. The expressions of miR‐383 and Wnt1 protein were detected in lung cancer tissues and cells by quantitative real‐time polymerase chain reaction (qRT‐PCR) and Western blot analysis. After the transfection of miR‐383 mimics, si‐Wnt1 or miR‐383+Wnt1, the viability and apoptosis of NSCLC cells were detected by cell counting kit‐8 and terminal deoxynucleotidyl transferase–mediated dUTP nick‐end labeling, respectively. The interaction between miR‐383 and Wnt1 was investigated b y luciferase activity and Western blot analysis. Cells stably transfected with miR‐383 mimics were inoculated into the right axillary of nude mice by subcutaneous injection. The tumor volume and weight were measured, and the expressions of miR‐383, Wnt1, β‐catenin, and cyclin D1 were detected by qRT‐PCR and Western blot analysis. The expression of miR‐383 was significantly decreased, and the level of Wnt1 was significantly increased (P <  0.05) in lung cancer tissues and cells. Upregulation of miR‐383 or inhibition of Wnt1 expression inhibited the cell viability and induce apoptosis in NSCLC cells. Moreover, Wnt1 was the target gene of miR‐383, and its overexpression weakened the regulatory effect of miR‐383 on cell viabilit y and apoptosis in NSCLC cells. Besides, the addition of miR‐383 de...

Source: Journal of Cellular Biochemistry - November 13, 2018 Category: Biochemistry Authors: Biao Gu, Jipeng Wang, Yaqi Song, Qi Wang, Qingquan Wu Tags: RESEARCH ARTICLE Source Type: research