NOTCH1 regulates the proliferation and migration of bladder cancer cells by cooperating with long non ‐coding RNA HCG18 and microRNA‐34c‐5p

This study focused on the mechanism by which NOTCH1 affects the biological behaviors of BCa cells. According to the experimental results of quantitative real ‐time polymerase chain reaction, NOTCH1 was dysregulated in BCa tissues and cell lines. The prognostic value of NOTCH1 for the patients with BCa was determined using the Kaplan‐Meier method. Mechanism investigations revealed that NOTCH1 is a target of miR‐34c‐5p in BCa. Furthermore, microarr ay analysis was used to find the dysregulated long noncoding RNAs (lncRNA), which can bind with miR‐34c‐5p. Mechanism experiments further demonstrated the rationality of the HCG18‐miR‐34c‐5p‐NOTCH1 pathway. Functional assays were then applied to validate the inhibitory influences of NOT CH1 on the proliferation and migration of BCa cells. Furthermore, the inhibitory effects of NOTCH1 could be affected by miR‐34c‐5p or lncRNA HCG18. All findings in this study revealed that NOTCH1 suppresses the BCa progression by cooperating with lncRNA HCG18 and miR‐34c‐5p.

Source: Journal of Cellular Biochemistry - November 13, 2018 Category: Biochemistry Authors: Zhihui Xu, Banggao Huang, Qi Zhang, Xiang He, Haibin Wei, Dahong Zhang Tags: RESEARCH ARTICLE Source Type: research