Investigating the role of CRIPTO ‐1 (TDGF‐1) in glioblastoma multiforme U87 cell line

In this study, we sought to delineate the role of Cripto‐1 in facilitating pathogenesis, stemness, proliferation, invasion, migration, and angiogenesis in GBM. Taken together our results support a role for Cripto‐1 in the initiation, development, progression and maintenance of GBM pathogenesis. AbstractCripto ‐1 has been implicated in a number of human cancers. Although there is high potential for a role of Cripto‐1 in glioblastoma multiforme (GBM) pathogenesis and progression, few studies have tried to define its role in GBM. These studies were limited in that Cripto‐1 expression was not studied i n detail in relation to markers of cancer initiation and progression. Therefore, these correlative studies allowed limited interpretation of Criptos‐1's effect on the various aspects of GBM development using the U87 GBM cell line. In this study, we sought to delineate the role of Cripto‐1 in fac ilitating pathogenesis, stemness, proliferation, invasion, migration and angiogenesis in GBM. Our findings show that upon overexpressing Cripto‐1 in U87 GBM cells, the stemness markers Nanog, Oct4, Sox2, and CD44 increased expression. Similarly, an increase in Ki67 was observed demonstrating Cript o‐1's potential to induce cellular proliferation. Likewise, we report a novel finding that increased expression of the markers of migration and invasion, Vimentin and Twist, correlated with upregulation of Cripto‐1. Moreover, Cripto‐1 exposure led to VEGFR‐2 overexpression ...
Source: Journal of Cellular Biochemistry - Category: Biochemistry Authors: Tags: RESEARCH ARTICLE Source Type: research