CircRNA circPDSS1 promotes the gastric cancer progression by sponging miR ‐186‐5p and modulating NEK2

This study mainly researched the effects of circPDSS1 and its related genes in gastric cancer (GC). 2.It revealed part of a complex regulation system, circPDSS1/miR ‐186‐5p/NEK2 axis in GC, and provided a potential therapeutic target for GC treating. 3.Further research should be done to investigate the related genes in this regulation system. AbstractThe aim of this study is to investigate the regulatory mechanism of circPDSS1/miR ‐186‐5p/NEK2 axis on the viability and proliferation in gastric cancer (GC) cell line. Differentially expressed circRNAs, miRNAs, and mRNAs in GC tissues and paracarcinoma tissues were analyzed using gene chips GSE83521, GSE89143, and GSE93415. Then, the expression of circPDSS1, miR‐186‐5p, and NEK2 was analyzed via quantitative real‐time polymerase chain reaction (qRT‐PCR). Survival analysis was adopted to explore the association between the circPDSS1 expression and the prognosis of GC. The effect of circPDSS1 on GC cell cycle and apoptosis was verified with the flow cytometry. Ta rgeting relationships among circPDSS1, miR‐186‐5p, and NEK2 were predicted via bioinformatics analysis and demonstrated by the dual‐luciferase reporter assay. Our results showed that circPDSS1 and NEK2 were high‐expressed whereas miR‐186‐5p was low‐expressed in GC tissues and cells. Ci rcPDSS1 promoted GC cell cycle and inhibited apoptosis by sponging miR‐186‐5p, while miR‐186‐5p inhibited cell cycle and promoted apoptosis by target...
Source: Journal of Cellular Physiology - Category: Cytology Authors: Tags: ORIGINAL RESEARCH ARTICLE Source Type: research