Cyadox regulates the transcription of different genes by activation of the PI3K signaling pathway in porcine primary hepatocytes

1. Increased expressions of growth ‐related genes such as an epidermal growth factor (EGF) and insulin‐like growth factor‐1 (IGF‐1) in porcine primary hepatocytes treated with cyadox are mediated mainly through phosphoinositide 3‐kinase (PI3K) signaling pathway, potentially leading to enhanced cell growth.2. EGF might be the early response gene of cyadox, and a primary regulator of other genes expression such as IGF ‐1 and the defender against apoptotic death 1 (DAD1), playing an important role in cell proliferation promoted by cyadox.3. Cyadox may promote transcriptional levels of seven cyadox ‐related genes by different signaling pathways in porcine primary hepatocytes, mainly via PI3K signaling pathway. AbstractCyadox, a new derivative of quinoxalines, has been ascertained as an antibiotic with significant growth promoting, low poison, quick absorption, swift elimination, brief residual period, and noncumulative effect. Seven differential expressed genes, includingInsulin ‐like Growth Factor‐1 (IGF ‐1),Epidermal Growth Factor (EGF),Poly ADP ‐ribose polymerase (PARP),the Defender Against Apoptotic Death 1 (DAD1),Complement Component 3 (C3),Transketolase (TK) and aNew gene, were induced by cyadox in swine liver tissues by messenger RNA differential display reverse transcription polymerase chain reaction (DDRT ‐PCR) in our laboratory. However, the signal mechanism that cyadox altered these genes expression is not completely elucidated. The signaling p...
Source: Journal of Cellular Biochemistry - Category: Biochemistry Authors: Tags: RESEARCH ARTICLE Source Type: research