Adenosine protects pancreatic beta cells against apoptosis induced by endoplasmic reticulum stress

Adenosine increase MIN6 insulinoma cell survival during endoplasmic reticulum (ER) stress. Thus, adenosine may be considered as a possible pharmacological agent to decrease of ER stress in pancreatic beta cells. AbstractChronic exposure to high glucose induces endoplasmic reticulum (ER) stress in pancreatic beta cells (PBCs). The previous evidence showed that adenosine modulate PBCs viability and insulin secretion. The aim of this study was to evaluate possible involvement of adenosine in protection of MIN6 β‐cells from Tunicamycin (Tu)‐induced ER stress.MIN6 cells were cotreated with Tu and different concentrations of adenosine. Cell viability, proliferation, and apoptosis were evaluated using 3 ‐[4,5‐dimethylthiazol‐2‐yl]‐2,5 diphenyl tetrazolium bromide (MTT), 5‐bromo‐2′‐deoxyuridine (Brdu), and colony formation assays. Caspase‐12 activity was assayed using the fluorometric method. Thioflavin T (ThT) staining was used for the evaluation of protein aggregation. Insulin secretion was evaluated using specific an ELISA kit. Ca2+ mobilization assayed using Fura2/AM probe. BIP, CHOP, XBP ‐1, and XBP‐1s expression in both messenger RNA (mRNA) and protein levels were evaluated using the reverse transcription‐polymerase chain reaction (RT‐PCR) and Western blot analysis, respectively. Bcl‐2, p‐eIF2α/eIF2α, and GADD34 levels also determined with Western blot analysis.Adenosine protected MIN6 cells against Tu ‐induced ER stress in a dose‐depe...
Source: Journal of Cellular Biochemistry - Category: Biochemistry Authors: Tags: RESEARCH ARTICLE Source Type: research